DOSIMMUNE™ immunosuppressants in whole blood using LC-MS/MS

Medical device

  • Available for 500 or 1 000 analysis
  • The set is ready-to-use with all consumables
  • Internal standard with isotope labeled analytes synthesized by Alsachim
  • For LC-MS/MS application
  • Analysis time: < 2 min.

DOSIMMUNE™ (227-40101-55/227-40100-55) is registered as a CE/IVD device in the EEA. In other case, DOSIMMUNE™ is intendent for research use only, not for diagnostic.

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Number of analysis


DOSIMMUNE™ CE-IVD (500 analyses 227-40101-55 or 1000 analyses 227-40100-55) is used for Therapeutic drug monitoring (TDM) of the main immunosuppressant drugs, Tacrolimus, Sirolimus, Everolimus and Cyclosporin A. TDM is essential to allow regulating of the dosage according to drug concentration of each patients’ whole blood after organ transplantation. Recently, LC-MS/MS has been introduced in many laboratories as an alternative technology for TDM (besides Immunoassays).

Alsachim developed a reagent kit based on LC-MS/MS analytical method for the quantification of immunosuppressants drugs in whole blood: Cyclosporin A, Sirolimus, Everolimus and Tacrolimus. The DOSIMMUNE™ kit is an in vitro diagnostic device for the rapid and multiplex assay of immunosuppressants drugs for LC-MS, thanks to in-house production of stable isotope-labeled internal standards with deuterium, carbon-13. This ready-to-use kit is also for routine LC-MS/MS analysis of these immunosuppressants in whole blood sample.

In other case, DOSIMMUNE™ RUO (227-40101-58 (Europe) / 227-40101-91 (Japon) is provided for Research Use Only (RUO).

Note: individual kit components available in RUO version only.

DOSIMMUNE is a registered trademark of Alsachim, a Shimadzu Group Company. This page may contain references to products that are not available in your country. This product could be available for research use only depending of your country. Please contact us to check the availability of these products in your country.

Technical Data

Method of analysis: LC-MS/MS
Parameter: Cyclosporin A, Sirolimus, Everolimus, Tacrolimus
Analysis time: 1.4 min 
Limit of quantification: 
- Cyclosporin A: ≤5.0 µg/L 
- Everolimus: ≤0.5 µg/L
- Sirolimus: ≤0.5 µg/L
- Tacrolimus: ≤0.5 µg/L
- Cyclosporin A: up to 3000 µg/L
- Everolimus: up to 100 µg/L
- Sirolimus: up to 100 µg/L
- Tacrolimus: up to 100 µg/L
Specimen: in wole blood 
Storage: Samples can be stored for up to 7 days at +4°C +/-2°C. For longer storage, it is recommended to freeze the sample at -20°C +/-5°C after collection.
Sample preparation: 
- Transfer calibrator or control or human blood sample into a microtube
- Add reconstituted Internal standards pool
- Add extraction buffer
- Shake and centrifuge
- Extract the supernatant
- Insert in LC/MS/MS and analyze
Injection volume: 1–50 µL
Ionisation: ESI positive
Comment: DOSIMMUNE™ reagent kit is delivered with all documentation required to use properly the product (complete instructions Manual, safety data sheets, quality an analysis certificates, etc.)

Kit Composition

  • Mobile Phase A
  • Mobile Phase B
  • System Cleaning Phase
  • Extraction Buffer
  • Internal Standard Set
  • Calibrator Set
  • Control Set
  • Analytical & Trap Columns
  • Microtubes
  • LC Vials with low volume glass insert & caps

  • Tuning Mix labeled and unlabeled (optional)


  • DOSIMMUNE™ immunosuppressants in whole blood using LC-MS/MS chromatogramm
  • DOSIMMUNE™ immunosuppressants in whole blood using LC-MS/MS chromatogramm
  • DOSIMMUNE™ immunosuppressants in whole blood using LC-MS/MS chromatogramm
  • DOSIMMUNE™ immunosuppressants in whole blood using LC-MS/MS chromatogramm

Kit components & accessories